The four individuals charged include Miami-Luken's former president and compliance officer and two West Virginia pharmacists, the release said.
The wholesaler distributed 2.6 million hydrocodone tablets and 2.3 million units of oxycodone to a pharmacy in a West Virginia town of only 1,400 people between 2011 and 2015, the Justice Department said.
The wholesaler also shipped more than 1.8 millions oxycodone tablets to a pharmacy that was under DEA investigation.
In April, two former executives of Rochester Drug Co-Operative were charged with illegally distributing opioids and conspiring to defraud the US Drug Enforcement Agency.
Miami-Luken sold drugs in Kentucky, Indiana, Ohio, Tennessee and West Virginia, according to the Justice Department.